Anti-HIV-1 clade B gp120 Recombinant Antibody (A32)
CAT#: MRO-022LC
Provided is a recombinant human anti-HIV-1 clade B monoclonal antibody. A32 specifically binds to gp120 and can be potentially used in the studies of acquired immunodeficiency syndrome (AIDS), a condition in humans characterized by clinical features including wasting syndromes, central nervous system degeneration and profound immunosuppression that results in life-threatening opportunistic infections and malignancies.
Tested Data
Published Data
WB
Figure 1 Anti-HIV-1 clade B gp120 Recombinant Antibody (MRO-022LC) in WB
Western blot analysis was performed with 1 µg (lane 1), 2 µg(lane 2) recombinant HIV-1 Env onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with MRO-022LC at a dilution of 1:250 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:2,000 for one hour. Chemiluminescent was detected.
DB
Figure 2 Anti-HIV-1 clade B gp120 Recombinant Antibody (MRO-022LC) in Dot Blot
Dot Blot analysis of MRO-022LC was performed by coating with HIV-1 Env antigen.
MRO-022LC incubation concentration: 4 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 3 Anti-HIV-1 clade B gp120 Recombinant Antibody (MRO-022LC) in ELISA
ELISA analysis of MRO-022LC was performed by coating with recombinant HIV-1 Env antigen. Then blocked with BSA, and incubated with MRO-022LC at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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WB
Figure 1. SDS-PAGE and Western blot analysis of unfractionated and FPLC-fractionated A32-rgp120 complexes.
Liao, H. X., Alam, S. M., Mascola, J. R., Robinson, J., Ma, B., Montefiori, D. C., ... & Haynes, B. F. (2004). Immunogenicity of constrained monoclonal antibody A32-human immunodeficiency virus (HIV) Env gp120 complexes compared to that of recombinant HIV type 1 gp120 envelope glycoproteins. Journal of virology, 78(10), 5270-5278.
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Specifications
- Host Species
- Human
- Type
- Human antibody
- Specificity
- HIV-1 clade B
- Clone
- A32
- Applications
- WB
- Related Disease
- AIDS
Applications
- Application Notes
- The A32 antibody has been reported in applications of WB.
It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated in specific assays. For details, refer to Published Data.
Target
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Specific Gp120 Detection
We used the Anti-HIV-1 clade B gp120 antibody (Clone A32) for Western Blotting. It specifically detected the gp120 glycoprotein. This antibody is a great resource for our research into the envelope glycoproteins of HIV-1 clade B.
High Quality Recombinant
The recombinant nature of this human monoclonal antibody ensures consistency. We have used it to study HIV-1 attachment mechanisms. It binds specifically to gp120, which is critical for our viral entry research projects.
Q&As
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What viral subtype does this antibody target?
A: This antibody specifically binds to gp120 of HIV-1 clade B, a glycoprotein exposed on the surface of the HIV envelope that is essential for virus entry into cells.
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What application is reported for this antibody?
A: This antibody (Clone A32) has been reported for use in Western Blot (WB) to detect the presence of HIV-1 clade B gp120 protein in samples.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MRO-022LC, RRID: AB_3714037)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Clone A32"
- CAT
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See other products for "HIV-1 clade B gp120"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-022LC-S(P) | Anti-HIV-1 clade B gp120 Recombinant Antibody scFv Fragment (A32) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-022LC-F(E) | Anti-HIV-1 clade B gp120 Recombinant Antibody Fab Fragment (A32) | ELISA | Human antibody |
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